Spontaneous anti-nuclear antibody (ANA) production in sex-matched and age-matched Crp knockout and control wild-type C57BL/6 mice. Open columns, ANA-negative mice with titre 1/80. (a) 3 months of age; (b) 6 months; © 9 months. Female Crp knockout mice had significantly more ANA by Fisher's exact test at 9 months (P = 0.03) and at 12 months (P = 0.002).much more susceptible to.Cence as described.Bacterial infectionStreptococcus pneumoniae isolates were from clinical pneumococcal infection cases or carriers, and from type cultures, and were typed, cultured and 예스카지노쿠폰 quantified by standard methods.
Mouse infection studies were conducted as previously described9 in sex-matched and 코인카지노쿠폰 closely age-matched groups of adult Crp knockout and wild-type control C57BL/6 mice, and were humanely killed at 72 hr.Non-infectious challengesC-reactive protein may have a role in preventing ANA formation12?4 and spontaneous ANA production became significantly greater among female but not male Crp knockouts at 9 months of age ( mice with ANA positive at 1 : 80 serum dilution, n = 22 per group, P = 0.03 by Fisher's exact test) and 12 months of age (P = 0.002, 우리카지노쿠폰 n = 21) (Fig.
2). A Crp transgenic study is required to determine whether this modest effect is indeed due to CRP deficiency because the sle1 locus, 예스카지노 which controls ANA production, is adjacent to the Crp gene on distal mouse chromosome 1. However, the response to immunization with apoptotic thymocytes did not differ between wild-type and Crp knockout mice (not shown).Study approvalAll mouse experiments were fully compliant with UK Home Office regulations, approved by the UCL Institutional Review Board.?2014 The Authors.
Immunology published by John Wiley Sons Ltd. Immunology, 142, 414?J. Paul Simons et al.(a) 60 (a)15 40 No. of mice 20 5 0 [SAP] mg/lFemaleMaleMale Crp+/+FemaleMale Crp??Female(b) 25 (b) 20 20 15 [SAA] mg/l No. of mice Female Male0 0 Male Crp +/+ © 25 Female Male Crp ??FemaleFigure 1.
Baseline concentrations of acute-phase proteins in sex and age matched Crp knockout and control wild-type C57BL/6 mice. Mean (SD), n = 7 per group, for (a) serum amyloid P component (SAP) and (b) serum amyloid A protein (SAA).Consistent with our previous finding that transgenic human CRP expression did not protect mice against the toxicity of Gram-negative bacterial lipopolysaccharide,15 there was no significant difference between Crp knockouts and wild-type mice in weight loss and clinical score or lethality (Fig.
3) produced by intraperitoneal injection of endotoxin at 10, 20 or 40 mg/kg. Local Shwartzman reactions induced by intradermal injection of endotoxin from Salmonella typhimurium, or from Haemophilus influenzae strains with and without phosphocholine expression, also did not differ between wild-type and 크레이지슬롯 Crp knockouts.No. of mice0 Male Crp +/+ Female Male Crp ??FemalePneumococcal infectionC-reactive protein-deficient mice were dramatically more susceptible than age- and 오바마카지노 sex-matched wild-type C57BL/6 controls to lethal infection after intraperitoneal inoculation of two independent clinical isolates of virulent type 6B S.
pneumoniae (strains Tz7712-2 and RF200) (Fig. 4a, b), with LD50 values of <10 and about 106 organisms, respectively (Fig. 4c). Lethality corresponded exactly with viable bacterial counts in the blood at 20 hr after inoculation; only mice with <104 colony-forming units per ml survived at 72 hr.